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He aha nga mahi o te kete ELISA?
2022-12-23
Ko te kete ELISA he mea hangai i runga i te wahanga totoka o te antigen, antibody ranei me te tapanga o te antigen, antibody ranei. Ko te antigen, antibody ranei kua herea ki te mata o te kaikawe totoka ka mau tonu tana mahi mate mate, a, ko te enzyme kua tapaina he antigen, antibody ranei e mau tonu ana ana mahi mate mate me te mahi enzyme. I te wa o te whakatau, ko te tauira i raro i te whakamatautau (e ine ai te antibody, antigen ranei) ka tauhohe ki te antigen, antibody ranei i runga i te mata o te kawe totoka. Ko te matatini antigen-antibody i hangaia i runga i te kawe totoka ka wehea mai i etahi atu matū i roto i te wai ma te horoi.
Ka taapirihia he antigens, he paturopi ranei kua tohua e te Enzyme, ka herea ki te kawe totoka ma te tauhohenga. I tenei wa, ko te nui o te enzyme i roto i te wahanga totoka e rite ana ki te nui o te matū o te tauira. Whai muri i te taapiri i te tïpako o te tauhohenga o te whana, ka whakakorikoria te tïpako e te whakïma kia puta he hua tae. Ko te nui o te hua e hono tika ana ki te nui o te matū i whakamatauria i roto i te tauira, na reira ka taea te mahi tātaritanga ine, ine ranei i runga i te hohonu o te tae.
Ko te kaha catalytic kaha o nga whākōkī ka whakakaha autaki i nga hua o te urupare aukati, na te mea he tino tairongo te whakamatautau. Ka taea te whakamahi i te ELISA ki te whakatau i nga antigens, engari ka taea hoki te whakamahi hei whakatau i nga antibodies.
Nga maapono taketake o te kete ELISA
Ka whakamahia e ia te tauhohenga motuhake o te antigen me te antibody hei hono i te ahanoa ki te whākōkī, kātahi ka puta te tauhohenga tae i waenga i te whākōkī me te tïpako hei whakatau ine. Ko te mea hei ine he antibody, antigen ranei.
E toru nga reagents e tika ana mo tenei tikanga whakatau:
â Wāhanga totoka antigen, antibody ranei (whakahau ira)
â¡ Te whakaahuru kua tapaina he antigen, antibody ranei (tohu)
⢠tïpako mo te mahi enzyme (kaiwhakawhanake tae)
I roto i te inenga, ka herea tuatahi te antigen (antibody) ki te kawe totoka, engari ka mau tonu tana mahi aukati, katahi ka taapirihia he conjugate (tohu) o te antibody (antigen) me te enzyme, e mau tonu ana tana mahi aukati me te enzyme. mahi. Ina tauhohe te conjugate ki te antigen (antibody) i runga i te kaikawe totoka, ka taapirihia te tïpako e rite ana ki te enzyme. Arā, te hydrolysis catalytic, REDOX tauhohenga me te tae.
Ka taapirihia he antigens, he paturopi ranei kua tohua e te Enzyme, ka herea ki te kawe totoka ma te tauhohenga. I tenei wa, ko te nui o te enzyme i roto i te wahanga totoka e rite ana ki te nui o te matū o te tauira. Whai muri i te taapiri i te tïpako o te tauhohenga o te whana, ka whakakorikoria te tïpako e te whakïma kia puta he hua tae. Ko te nui o te hua e hono tika ana ki te nui o te matū i whakamatauria i roto i te tauira, na reira ka taea te mahi tātaritanga ine, ine ranei i runga i te hohonu o te tae.
Ko te kaha catalytic kaha o nga whākōkī ka whakakaha autaki i nga hua o te urupare aukati, na te mea he tino tairongo te whakamatautau. Ka taea te whakamahi i te ELISA ki te whakatau i nga antigens, engari ka taea hoki te whakamahi hei whakatau i nga antibodies.
Nga maapono taketake o te kete ELISA
Ka whakamahia e ia te tauhohenga motuhake o te antigen me te antibody hei hono i te ahanoa ki te whākōkī, kātahi ka puta te tauhohenga tae i waenga i te whākōkī me te tïpako hei whakatau ine. Ko te mea hei ine he antibody, antigen ranei.
E toru nga reagents e tika ana mo tenei tikanga whakatau:
â Wāhanga totoka antigen, antibody ranei (whakahau ira)
â¡ Te whakaahuru kua tapaina he antigen, antibody ranei (tohu)
⢠tïpako mo te mahi enzyme (kaiwhakawhanake tae)
I roto i te inenga, ka herea tuatahi te antigen (antibody) ki te kawe totoka, engari ka mau tonu tana mahi aukati, katahi ka taapirihia he conjugate (tohu) o te antibody (antigen) me te enzyme, e mau tonu ana tana mahi aukati me te enzyme. mahi. Ina tauhohe te conjugate ki te antigen (antibody) i runga i te kaikawe totoka, ka taapirihia te tïpako e rite ana ki te enzyme. Arā, te hydrolysis catalytic, REDOX tauhohenga me te tae.
Ko te whakamarumaru o te tae ka puta he rite ki te nui o te antigen (antibody) hei ine. Ko tenei hua tae ka kitea e te kanohi tahanga, karu whatu, karu irahiko, ka taea hoki te ine ma te spectrophotometer (taputapu tapanga enzyme). He ngawari te tikanga, he watea, he tere me te motuhake.
Tuhinga o mua:Whakaaturanga o Elisa Plate
